anndata.concat

anndata.concat(adatas, *, axis=0, join='inner', merge=None, uns_merge=None, label=None, keys=None, index_unique=None, fill_value=None, pairwise=False)

Concatenates AnnData objects along an axis.

See the concatenation section in the docs for a more in-depth description.

Warning

This function is marked as experimental for the 0.7 release series, and will supercede the AnnData.concatenate() method in future releases.

Parameters

adatas : Collection[AnnData] | MappingUnion[Collection[AnnData], Mapping[str, AnnData]]

The objects to be concatenated. If a Mapping is passed, keys are used for the keys argument and values are concatenated.

axis : {0, 1}Literal[0, 1] (default: 0)

Which axis to concatenate along.

join : {‘inner’, ‘outer’}Literal[‘inner’, ‘outer’] (default: 'inner')

How to align values when concatenating. If “outer”, the union of the other axis is taken. If “inner”, the intersection. See concatenation for more.

merge : {‘same’, ‘unique’, ‘first’, ‘only’} | Callable | NoneUnion[Literal[‘same’, ‘unique’, ‘first’, ‘only’], Callable, None] (default: None)

How elements not aligned to the axis being concatenated along are selected. Currently implemented strategies include:

• None: No elements are kept.

• "same": Elements that are the same in each of the objects.

• "unique": Elements for which there is only one possible value.

• "first": The first element seen at each from each position.

• "only": Elements that show up in only one of the objects.

uns_merge : {‘same’, ‘unique’, ‘first’, ‘only’} | Callable | NoneUnion[Literal[‘same’, ‘unique’, ‘first’, ‘only’], Callable, None] (default: None)

How the elements of .uns are selected. Uses the same set of strategies as the merge argument, except applied recursively.

label : str | NoneOptional[str] (default: None)

Column in axis annotation (i.e. .obs or .var) to place batch information in. If it’s None, no column is added.

keys : Collection | NoneOptional[Collection] (default: None)

Names for each object being added. These values are used for column values for label or appended to the index if index_unique is not None. Defaults to incrementing integer labels.

index_unique : str | NoneOptional[str] (default: None)

Whether to make the index unique by using the keys. If provided, this is the delimeter between “{orig_idx}{index_unique}{key}”. When None, the original indices are kept.

fill_value : Any | NoneOptional[Any] (default: None)

When join="outer", this is the value that will be used to fill the introduced indices. By default, sparse arrays are padded with zeros, while dense arrays and DataFrames are padded with missing values.

pairwise : bool (default: False)

Whether pairwise elements along the concatenated dimension should be included. This is False by default, since the resulting arrays are often not meaningful.

Notes

Warning

If you use join='outer' this fills 0s for sparse data when variables are absent in a batch. Use this with care. Dense data is filled with NaN.

Examples

Preparing example objects

>>> import anndata as ad, pandas as pd, numpy as np
>>> from scipy import sparse
>>> a = ad.AnnData(
...     X=sparse.csr_matrix(np.array([[0, 1], [2, 3]])),
...     obs=pd.DataFrame({"group": ["a", "b"]}, index=["s1", "s2"]),
...     var=pd.DataFrame(index=["var1", "var2"]),
...     varm={"ones": np.ones((2, 5)), "rand": np.random.randn(2, 3), "zeros": np.zeros((2, 5))},
...     uns={"a": 1, "b": 2, "c": {"c.a": 3, "c.b": 4}},
... )
>>> b = ad.AnnData(
...     X=sparse.csr_matrix(np.array([[4, 5, 6], [7, 8, 9]])),
...     obs=pd.DataFrame({"group": ["b", "c"], "measure": [1.2, 4.3]}, index=["s3", "s4"]),
...     var=pd.DataFrame(index=["var1", "var2", "var3"]),
...     varm={"ones": np.ones((3, 5)), "rand": np.random.randn(3, 5)},
...     uns={"a": 1, "b": 3, "c": {"c.b": 4}},
... )
>>> c = ad.AnnData(
...     X=sparse.csr_matrix(np.array([[10, 11], [12, 13]])),
...     obs=pd.DataFrame({"group": ["a", "b"]}, index=["s1", "s2"]),
...     var=pd.DataFrame(index=["var3", "var4"]),
...     uns={"a": 1, "b": 4, "c": {"c.a": 3, "c.b": 4, "c.c": 5}},
... )

Concatenating along different axes

>>> ad.concat([a, b]).to_df()
    var1  var2
s1   0.0   1.0
s2   2.0   3.0
s3   4.0   5.0
s4   7.0   8.0
>>> ad.concat([a, c], axis=1).to_df()
    var1  var2  var3  var4
s1   0.0   1.0  10.0  11.0
s2   2.0   3.0  12.0  13.0

Inner and outer joins

>>> inner = ad.concat([a, b])  # Joining on intersection of variables
>>> inner
AnnData object with n_obs × n_vars = 4 × 2
    obs: 'group'
>>> (inner.obs_names, inner.var_names)  
(Index(['s1', 's2', 's3', 's4'], dtype='object'),
Index(['var1', 'var2'], dtype='object'))
>>> outer = ad.concat([a, b], join="outer") # Joining on union of variables
>>> outer
AnnData object with n_obs × n_vars = 4 × 3
    obs: 'group', 'measure'
>>> outer.var_names
Index(['var1', 'var2', 'var3'], dtype='object')
>>> outer.to_df()  # Sparse arrays are padded with zeroes by default
    var1  var2  var3
s1   0.0   1.0   0.0
s2   2.0   3.0   0.0
s3   4.0   5.0   6.0
s4   7.0   8.0   9.0

Keeping track of source objects

>>> ad.concat({"a": a, "b": b}, label="batch").obs
   group batch
s1     a     a
s2     b     a
s3     b     b
s4     c     b
>>> ad.concat([a, b], label="batch", keys=["a", "b"]).obs  # Equivalent to previous
   group batch
s1     a     a
s2     b     a
s3     b     b
s4     c     b
>>> ad.concat({"a": a, "b": b}, index_unique="-").obs
     group
s1-a     a
s2-a     b
s3-b     b
s4-b     c

Combining values not aligned to axis of concatenation

>>> ad.concat([a, b], merge="same")
AnnData object with n_obs × n_vars = 4 × 2
    obs: 'group'
    varm: 'ones'
>>> ad.concat([a, b], merge="unique")
AnnData object with n_obs × n_vars = 4 × 2
    obs: 'group'
    varm: 'ones', 'zeros'
>>> ad.concat([a, b], merge="first")
AnnData object with n_obs × n_vars = 4 × 2
    obs: 'group'
    varm: 'ones', 'rand', 'zeros'
>>> ad.concat([a, b], merge="only")
AnnData object with n_obs × n_vars = 4 × 2
    obs: 'group'
    varm: 'zeros'

The same merge strategies can be used for elements in .uns

>>> dict(ad.concat([a, b, c], uns_merge="same").uns)
{'a': 1, 'c': {'c.b': 4}}
>>> dict(ad.concat([a, b, c], uns_merge="unique").uns)
{'a': 1, 'c': {'c.a': 3, 'c.b': 4, 'c.c': 5}}
>>> dict(ad.concat([a, b, c], uns_merge="only").uns)
{'c': {'c.c': 5}}
>>> dict(ad.concat([a, b, c], uns_merge="first").uns)
{'a': 1, 'b': 2, 'c': {'c.a': 3, 'c.b': 4, 'c.c': 5}}

Return type

AnnData